Single-Cell RNA-Seq Analysis: Diffusion Pseudotime Reconstruction of Single-Cell Lineage Branching
Introduction Using single-cell data, cells arranged along trajectories can be calculated, allowing for an unbiased study of cellular dynamics. Since 2014, more than 50 trajectory inference methods have been developed, each with its own set of methodological characteristics. This article will use two forms of pseudotime analysis (Diffusion Components and Diffusion Pseudotime) to infer the … Read more